• Qualifications: MSc, Ph.D
  • Department: Department of Biotechnology & Applied Genetics
  • Designation:Assistant Professor, Dean of Sciences & Head of Research
  • Years of Experience: Post-doctoral research – 5 years, Teaching – 6 years
  • Email: gireesh.bojanala@gmail.com

Academic Achievements

Awards & Recognitions
  1. Labex CelTisPhyBio travel grant award  – 2017
  2. Labex CelTisPhyBio postdoctoral award  – 2016
  3. Qualified for National Eligibility Test-Lectureship (NET-LS), conducted by Council of   Scientific and Industrial Research, Govt. of India  – 2002 & 2003
  4. Secured 4th position among the class during M.Sc Biotechnology program – 2002
  5. Secured1st ranks in M.Sc qualifying entrance examinations in Biotechnology, Biochemistry and Virology programs conducted by Sri Venkateswara University and Sri Krishnadevaraya University in India -2000
Invited Talks
  1. “C. elegans as a model organism for developmental genetics” at Ph.D student seminar series “Bugs and Beasts” Prague  – 2009
  2. “Forward Genetic Tools available for C. elegans research” at Ph.D student seminar series “Heavy Tools” Prague – 2010
  3. “C. elegans as a model organism for developmental genetics” at PhD retreat, Brno -2011
Research Experience
  1. Department of Molecular Reproduction, Development and Genetics,Indian Institute of Science, Bangalore, India.
    Post-doctoral Scholar, Jan -July 2018
    Varsha Singh Lab
    Title: Regulation of lipid droplets during immune response in C. elegans after E. faecalis infection
    For centuries, the common man notion is that fat is bad for health. But, scientists have recently discovered growing evidence that fat cells have a protective role during bacterial infection in mammals, and in wound healing process in fruit flies. In the present study, for the first time, we aim to dissect the role of lipid droplets during immune response in C. elegans,after infection with Gram-positive bacterium, Enterococcus faecalis. Our preliminary studies strongly suggest that lipid droplets are mobilized immediately after infection, and can help in eliciting immune response.Our long-term goal is to better understand the gene regulatory networks that link lipid homeostasis to host defensesin infection.In all, our findings can be further studied in humans to better understand the role of fat during host defense mechanisms, and can eventually help to identify novel therapeutics based on fat homeostasis.
  2. Laboratoire Physico-Chemie (UMR 168), Paris, France.
    Post-doctoral Scholar, Oct 2016-Dec 2017
    Julie Plastino Lab
    Title: Action dynamics in cell invasion and migration during C. elegans development
    Analyzed actin dynamics during a developmental event, Anchor Cell (AC) invasion in C. elegans through transgenesis, RNAi, cloning, and microscopy techniques. Specifically, studied the role of actin nucleator protein Arp 2/3, and its upstream regulators Wiskott–Aldrich syndrome protein (WASP) and WASP-family verprolin-homologous protein (WAVE), during invadopodia formation from AC and subsequent breaching of Basement Membrane (BM) that bridges neighboring vulva cells. The findings from this study will help to better understand cell invasion during cancer metastasis due to the conserved role of actin regulators in nematodes and humans.
  3. Tata Institute of Fundamental Research, Mumbai, India
    Post-doctoral trainee, Dec 2015- Sep 2016
    Sandhya P Koushika Lab
    Title: Imaging retrogradely transported vesicles through I-switch in C. elegans
    This work is part of a collaborative effortthat aims to develop a method to exclusively track retrogradely moving synaptic vesicle proteins during neuronal transport by specialized DNA nanomachine, I-switch. For this purpose, I analyzed expression pattern of transgenic lines containing psnb-1::SNB-1::9E, which assist in binding I-switch (SNB-1: Synaptobrevin-1).  Furthermore, validated the functionality of these transgene to rescue snb-1 hypomorhic and null mutant phenotypes.
  4. PennState University, State College, PA
    Visiting Scholar, Jan 2013-Mar 2015
    Wendy Hanna-Rose Lab
    Title: Role of EGL-26 during vulva organogenesis
    The LRAT (Lecithin Retinol Acyl Transferase) family protein, EGL-26 controls vulva morphogenesis through maintaining VulF derived dorsal lumen. With an aim to dissect the mechanistic role of EGL-26, I analyzed actin organization in VulF lumen in wtand egl-26 mutants throughlifeAct::GFPreporter. Abnormal actin organization within VulF was seen in egl-26 mutants, suggesting a cross talk between EGL-26 and cytoskeleton during vulva lumen formation. I also dissected egl-26 promoter for cis-regulatory elements and TF binding sites through bioinformatics tool, LASAGNA.Title: Rotational manipulation of worms through acoustic waves.
    In a collaborative effort with Penn State bioengineering department, I established ARM (Acoustic Rotational Manipulation) method to precisely rotate C. elegans in microfluidic devices. This method helped to investigate neuronal pattering of ALA dendrites and reproductive pathologies in worms.
  5. Biology Center, ASCR, Ceske Budejovice, Czech Republic Ph.D Candidate, 2008-2015
    Masako Asahina Lab
    Title: Modulation of C. elegans vulva organogenesis by nuclear hormone receptor NHR-25
    During my Ph.D I analyzed the role of conserved nuclear hormone receptor NHR-25 during vulva formation in C. elegans through genetic, cellular, and molecular techniques. Graded expression of NHR-25 was seen during vulva cell fate specifications and migrations. In vitro and in vivo sumoylation assays identified that a proper ratio of sumoylated to un-sumoylated NHR-25 specifies 30 cell fates. In addition, nhr-25(RNAi) and nhr-25(ku217) studies identified a pivotal role for NHR-25 during vulva cell migrations, fusions and morphogenesis. Altered gene expression pattern of EGF/LIN-3 and HOX/LIN-39 were seen in nhr-25(lf)within vulva. Observed genetic interaction with smp-1/plx-1 pathway and nhr-25 during collective vulva cell migrations.
  6. McMaster University, Ontario, Canada.
    MS candidate, Jan 2005-May 2008
    Bhagwati P Gupta Lab
    Title: Genetic analysis of pry-1/axin in Caenorhabditis briggsae
    During the study I aimed to analyze and compare the role of pry-1/Axin during vulva development in C. elegans and C. briggsae. Quantified vulva phenotypes in Cbr-pry-1 through vulva cell fate marker expression, inductions and lineage analysis. Carried out genetic interaction of cbr-pry-1 with canonical Wnt signaling components, cbr-bar-1cbr-pop-1 and Hox cbr-lin-39. Further comparative analysis with Ce-pry-1 identified novel role of Wnt signaling in specifying of 20 cell fates.
Teaching Activities
  1. Instructor, Biology 430/BMB430, Fall 2014
    Penn State University, USA.
    Planned and conducted weekly reading assignments, lecture series, quizzes and exams for BMB-BIOL 430. Assisted and monitored learning assistants and group leaders during the course. Interacted with students on review sessions, weekly office hours and individual meetings to assist them in exams, quizzes and problem sets.  Advised students on their future career prospects.
  2. Teaching Assistant, Practical course in Developmental Biology, May 2009 -2012
    University of South Bohemia, CZ.
    Involved in the teaching/lab activities to explain “C. elegans as a model organism for developmental genetics”
  3. Teaching Assistant, Jan 2005-Mar 2007
    Department of Biology, McMaster University, Canada.
    Conducted labs and reading assignments as undergraduate teaching assistant for 1A03 (Introductory biology) and 3MO3 (Developmental biology).
  4. Teaching Assistant, May 2003-Apr 2004
    Sri Venkateswara University, Tirupati, India.
Publications
  1. IRodrigo Cáceres, Nagagireesh Bojanala, Laura C. Kelley, Jes Dreier, John Manzi, Fahima Di            Federico, Qiuyi Chi, Thomas Risler, Ilaria Testa, David R. Sherwood and Julie PlastinoForces  drive  basement membrane invasion in Caenorhabditis elegans.   (in revision) PNAS 2018.IF 9.5
  2. Ahmed D, Ozcelik A, Bojanala N, Nama N, Upadhyay A, Chen Y, Hanna-Rose W, Huang TJ.  Rotational manipulation of single cells and organisms using acoustic waves. Nat Commun. 2016 Mar 23;7:11085. doi: 10.1038/ncomms11085.IF 12.35
  3. Jordan D. Ward*Nagagireesh Bojanala*, Teresita Bernal, Kaveh Ashrafi,Masako Asahina, and Keith R. Yamamoto. Sumoylated NHR-25/NR5A regulates cell fate during C. elegans vulval development.Published: PlosGenetics December 12, 2013. DOI: 10.1371/journal.pgen.1003992. * Equal Contribution.IF 5.54
  4. Devika Sharanya, Bavithra Thillainathan, Sujatha Marri, Nagagireesh Bojanala, Stephane Flibotte, Donald G. Moerman, Robert H. Waterston, and Bhagwati P Gupta.Genetic Control of Vulval Development in Caenorhabditis briggsae. G3(Bethesda) 2012.doi: 10.1534/g3.112.004598. IF 2.86
  5. Seetharaman A., Cumbo P., Bojanala N., Gupta B.P. (2010).  Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae.Developmental Biology 346: 128–139. IF 3.26
Conferences
  1. June 21-25, 2017, 21st Intenational C. elegans meeting, UCLA, USA.“Actin cytoskeleton dynamics in anchor cell invasion”R. Caceres1N. Bojanala1, L. Kelley2, D. Sherwood2, and J. Plastino1(Poster)
  2. Jan 20, 2017, Vermidi XX, ENS Lyon, France.“Actin cytoskeleton in cell invasion and migration in Caenorhabditis elegans vulval development” Nagagireesh Bojanala, Rodrigo Caceres, and Julie Plastino (Poster).
  3. 3) June 26-30, 2013, 19th International C. elegans Meeting, UCLA.“Dissecting the mechanistic insights through which EGL-26 controls vulva organogenesis” Nagagireesh Bojanala, Avni upadhyay, Hongliu Sun, Matt Crook, ArianaDetwiler, Nishat Seraj, Sarah Chang, Jimmy Goncalves, Ryan Fine, Nick Serra, and Wendy Hanna-Rose (Poster)
  4. 4) June 7 – 10, 2012, University of Wisconsin, Madison. C. elegans Development, Cell Biology, & Gene Expression Meeting.C. elegans nuclear hormone receptor, nhr-25 regulates vulval terminal cell properties and migrations during development”Nagagireesh Bojanala, Marek Jindraand Masako Asahina (Poster).
  5. 5) June 22-26, 2011, 18 th International Worm Meeting, UCLA.“Sumoylation of C. elegans nuclear receptor NHR-25 promotes proper organogenesis” Jordan D. Ward, Nagagireesh Bojanala, Teresita Bernal, Marek Jindra, Kaveh Ashrafi, Keith R. Yamamoto, Masako Asahina.“Vulval organogenesis involves an interaction between SMO-1 and the nuclear receptor NHR-25” Jordan D. Ward, Nagagireesh Bojanala, Teresita Bernal, Marek Jindra, Kaveh Ashrafi, Keith R. Yamamoto, Masako Asahina.
  6. Sept 16-20, 2011. EMBO conference series, Sitges, Spain.Nuclear Receptors: From Molecular Mechanism to Health and Disease.“Sumoylation of C. elegans nuclear receptor NHR-25 promotes proper organogenesis”Jordan D. Ward, Nagagireesh bojanala, Teresita Bernal, Marek Jindra, Kaveh Ashrafi, Keith R. Yamamoto, Masako Asahina.
  7. June 17-20, 2010.  C. elegans EMBO conference series, Heidelberg, Germany.“Regulation of the nuclear receptor NHR-25 by sumoylation during C. elegans vulva formation”. Nagagireesh Bojanala, Jordan D. Ward , Teresita Bernal, Marek Jindra, Kaveh Ashrafi, Keith R. Yamamoto, Masako Asahina(Poster) .
  8. Aug 31st-Sept 4th, 2010, CSHL Nuclear Receptors and Disease Conference, Cold Spring Harbor Laboratories, NY, USA.“Sumoylation of C. elegans nuclear receptor NHR-25 promotes proper organogenesis”Jordan D. Ward, Nagagireesh Bojanala, Teresita Bernal, Marek Jindra, Kaveh Ashrafi, Keith R. Yamamoto, Masako Asahina.